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mouse il 1 β elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd mouse il 1 β elisa kit
    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
    Mouse Il 1 β Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+1+%CE%B2/Mouse+IL-1%CE%B2+High+Sensitivity+ELISA+Kit/pmc13084338-43-0-6
    Average 95 stars, based on 844 article reviews
    mouse il 1 β elisa kit - by Bioz Stars, 2026-09
    95/100 stars

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    1) Product Images from "TREM2 sustains glucose metabolic homeostasis to drive antibacterial defense during sepsis"

    Article Title: TREM2 sustains glucose metabolic homeostasis to drive antibacterial defense during sepsis

    Journal: iScience

    doi: 10.1016/j.isci.2026.115415

    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
    Figure Legend Snippet: TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.

    Techniques Used: Injection, Clinical Proteomics, Comparison

    TREM2 deficiency triggers F4/80 + peritoneal macrophages from pyroptosis (A) Pyroptosis of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (B) Immunoblot of caspase-1 and IL-1 β in PLF at 9 h after the intraperitoneal injection of E. coli . Representative image from three independent experiments is shown. (C) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (D) Immunoblot of caspase-1, GSDMD, and IL-1 β in the cells and supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h. Representative image from three independent experiments is shown. (E) LDH level in the supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (G–H) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (I) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range, the p values were determined by Kruskal-Wallis test followed by Dunn’s post hoc test. (J) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 in the WT → WT group, n = 10 in other groups); the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, Peritoneal lavage fluid; LDH, lactic dehydrogenase; SN, supernatant.
    Figure Legend Snippet: TREM2 deficiency triggers F4/80 + peritoneal macrophages from pyroptosis (A) Pyroptosis of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (B) Immunoblot of caspase-1 and IL-1 β in PLF at 9 h after the intraperitoneal injection of E. coli . Representative image from three independent experiments is shown. (C) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (D) Immunoblot of caspase-1, GSDMD, and IL-1 β in the cells and supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h. Representative image from three independent experiments is shown. (E) LDH level in the supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (G–H) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (I) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range, the p values were determined by Kruskal-Wallis test followed by Dunn’s post hoc test. (J) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 in the WT → WT group, n = 10 in other groups); the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, Peritoneal lavage fluid; LDH, lactic dehydrogenase; SN, supernatant.

    Techniques Used: Injection, Western Blot, Incubation, Comparison, Clinical Proteomics

    TREM2 deficiency-mediated IL-1 β release promotes insulin production through islet IL-1R1 receptor (A) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (C) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (D) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 per group); the p values were analyzed by the Mantel-Cox test. (E) Plasma IL-1 β level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test at 9 h. (G) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (H) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as median ± interquartile range; the p values were determined by the Mann-Whitney U test. (I) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 16 in the Trem2 −/− → Il1r1 fl/fl Pdx1 -Cre group, n = 14 in the Trem2 −/− → Il1r1 fl/fl group), the p value was analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; CFU, colony-forming units.
    Figure Legend Snippet: TREM2 deficiency-mediated IL-1 β release promotes insulin production through islet IL-1R1 receptor (A) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (C) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (D) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 per group); the p values were analyzed by the Mantel-Cox test. (E) Plasma IL-1 β level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test at 9 h. (G) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (H) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as median ± interquartile range; the p values were determined by the Mann-Whitney U test. (I) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 16 in the Trem2 −/− → Il1r1 fl/fl Pdx1 -Cre group, n = 14 in the Trem2 −/− → Il1r1 fl/fl group), the p value was analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; CFU, colony-forming units.

    Techniques Used: Injection, Comparison, Clinical Proteomics, MANN-WHITNEY

    Glucose supplementation protects the host from lethal outcomes during sepsis (A) Blood glucose level in septic mice after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B-C) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (D) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by the Dunn’s post hoc test. (E) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), the p values were analyzed by the Mantel-Cox test. (F) Death of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, peritoneal lavage fluid; LDH, lactic dehydrogenase; CFU, colony-forming units.
    Figure Legend Snippet: Glucose supplementation protects the host from lethal outcomes during sepsis (A) Blood glucose level in septic mice after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B-C) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (D) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by the Dunn’s post hoc test. (E) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), the p values were analyzed by the Mantel-Cox test. (F) Death of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, peritoneal lavage fluid; LDH, lactic dehydrogenase; CFU, colony-forming units.

    Techniques Used: Injection, Saline, Comparison, Clinical Proteomics

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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels <t>of</t> <t>IL-1</t> β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.
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    TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.

    Journal: iScience

    Article Title: TREM2 sustains glucose metabolic homeostasis to drive antibacterial defense during sepsis

    doi: 10.1016/j.isci.2026.115415

    Figure Lengend Snippet: TREM2 regulates glucose homeostasis through controlling the release of IL-1β during sepsis (A) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (B) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 18 in the E. coli -injected Trem2 −/− and WT group, n = 5 in the PBS-injected Trem2 −/− and WT group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (C) Levels of IL-1 β and TNF-α in PLF at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in the PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (D) Plasma levels of IL-1 β and TNF-α at 9 h after the intraperitoneal injection of E. coli or PBS solution ( n = 10 in the E. coli -injected Trem2 fl/fl Lyz2- Cre group, n = 15 in the E. coli -injected Trem2 fl/fl group, n = 5 in PBS-injected Trem2 fl/fl Lyz2- Cre and Trem2 fl/fl group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (E) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (F) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range; the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (H) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), and the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PLF, peritoneal lavage fluid; CFU, colony-forming units.

    Article Snippet: Mouse IL-1 β ELISA kit , Liankebio , Cat# EK201BHS-96.

    Techniques: Injection, Clinical Proteomics, Comparison

    TREM2 deficiency triggers F4/80 + peritoneal macrophages from pyroptosis (A) Pyroptosis of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (B) Immunoblot of caspase-1 and IL-1 β in PLF at 9 h after the intraperitoneal injection of E. coli . Representative image from three independent experiments is shown. (C) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (D) Immunoblot of caspase-1, GSDMD, and IL-1 β in the cells and supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h. Representative image from three independent experiments is shown. (E) LDH level in the supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (G–H) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (I) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range, the p values were determined by Kruskal-Wallis test followed by Dunn’s post hoc test. (J) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 in the WT → WT group, n = 10 in other groups); the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, Peritoneal lavage fluid; LDH, lactic dehydrogenase; SN, supernatant.

    Journal: iScience

    Article Title: TREM2 sustains glucose metabolic homeostasis to drive antibacterial defense during sepsis

    doi: 10.1016/j.isci.2026.115415

    Figure Lengend Snippet: TREM2 deficiency triggers F4/80 + peritoneal macrophages from pyroptosis (A) Pyroptosis of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (B) Immunoblot of caspase-1 and IL-1 β in PLF at 9 h after the intraperitoneal injection of E. coli . Representative image from three independent experiments is shown. (C) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p value was determined by Student’s t test. (D) Immunoblot of caspase-1, GSDMD, and IL-1 β in the cells and supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h. Representative image from three independent experiments is shown. (E) LDH level in the supernatants of Trem2 −/− and WT peritoneal macrophages after incubation with heat-killed E. coli for 9 h ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (G–H) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (I) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 12 per group), data are presented as median ± interquartile range, the p values were determined by Kruskal-Wallis test followed by Dunn’s post hoc test. (J) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 in the WT → WT group, n = 10 in other groups); the p values were analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, Peritoneal lavage fluid; LDH, lactic dehydrogenase; SN, supernatant.

    Article Snippet: Mouse IL-1 β ELISA kit , Liankebio , Cat# EK201BHS-96.

    Techniques: Injection, Western Blot, Incubation, Comparison, Clinical Proteomics

    TREM2 deficiency-mediated IL-1 β release promotes insulin production through islet IL-1R1 receptor (A) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (C) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (D) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 per group); the p values were analyzed by the Mantel-Cox test. (E) Plasma IL-1 β level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test at 9 h. (G) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (H) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as median ± interquartile range; the p values were determined by the Mann-Whitney U test. (I) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 16 in the Trem2 −/− → Il1r1 fl/fl Pdx1 -Cre group, n = 14 in the Trem2 −/− → Il1r1 fl/fl group), the p value was analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; CFU, colony-forming units.

    Journal: iScience

    Article Title: TREM2 sustains glucose metabolic homeostasis to drive antibacterial defense during sepsis

    doi: 10.1016/j.isci.2026.115415

    Figure Lengend Snippet: TREM2 deficiency-mediated IL-1 β release promotes insulin production through islet IL-1R1 receptor (A) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (C) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 9 per group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by Dunn’s post hoc test. (D) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 9 per group); the p values were analyzed by the Mantel-Cox test. (E) Plasma IL-1 β level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. (F) Blood glucose level in mice after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test at 9 h. (G) Plasma insulin level at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as mean ± SEM, the p values were determined by Student’s t test. (H) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 10 per group), data are presented as median ± interquartile range; the p values were determined by the Mann-Whitney U test. (I) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 16 in the Trem2 −/− → Il1r1 fl/fl Pdx1 -Cre group, n = 14 in the Trem2 −/− → Il1r1 fl/fl group), the p value was analyzed by the Mantel-Cox test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; CFU, colony-forming units.

    Article Snippet: Mouse IL-1 β ELISA kit , Liankebio , Cat# EK201BHS-96.

    Techniques: Injection, Comparison, Clinical Proteomics, MANN-WHITNEY

    Glucose supplementation protects the host from lethal outcomes during sepsis (A) Blood glucose level in septic mice after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B-C) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (D) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by the Dunn’s post hoc test. (E) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), the p values were analyzed by the Mantel-Cox test. (F) Death of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, peritoneal lavage fluid; LDH, lactic dehydrogenase; CFU, colony-forming units.

    Journal: iScience

    Article Title: TREM2 sustains glucose metabolic homeostasis to drive antibacterial defense during sepsis

    doi: 10.1016/j.isci.2026.115415

    Figure Lengend Snippet: Glucose supplementation protects the host from lethal outcomes during sepsis (A) Blood glucose level in septic mice after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test at 9 h. (B-C) Plasma levels of IL-1 β and insulin at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (D) Bacterial load in the livers and spleens at 9 h after the intraperitoneal injection of E. coli ( n = 17 in the glucose-treated Trem2 −/− group, n = 16 in the glucose-treated WT group, n = 15 in the saline-treated Trem2 −/− group, n = 17 in the saline-treated WT group), data are presented as median ± interquartile range, the p values were determined by the Kruskal-Wallis test followed by the Dunn’s post hoc test. (E) Percentage survival of mice after the intraperitoneal injection of E. coli ( n = 12 per group), the p values were analyzed by the Mantel-Cox test. (F) Death of peritoneal macrophages at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, the p values were determined by one-way ANOVA with Tukey’s multiple comparison post hoc test. (G) LDH level in PLF at 9 h after the intraperitoneal injection of E. coli ( n = 6 per group), data are presented as mean ± SEM, and the p values were determined by Student’s t test. TREM2, triggering receptor expressed on myeloid cells 2; WT, wild type; PI, propidium iodide; PLF, peritoneal lavage fluid; LDH, lactic dehydrogenase; CFU, colony-forming units.

    Article Snippet: Mouse IL-1 β ELISA kit , Liankebio , Cat# EK201BHS-96.

    Techniques: Injection, Saline, Comparison, Clinical Proteomics